Permanent light microscopy slides of Eimeria nieschulzi oocysts.
نویسندگان
چکیده
منابع مشابه
On the status of Eimeria nieschulzi oocysts embedded in resin eleven years ago: a permanent method for preserving coccidian oocysts.
Sporulated oocysts of Eimeria nieschulzi that were fixed and mounted on glass slides in polymerized resin in 1976 are examined. Size, shape, and integrity of oocysts and sporocysts are compared to similar observations we made in 1977 and reported in 1978 (Journal of Parasitology 64: 163-164). Our conclusion is that the methods we reported on in 1978 provide one opportunity to produce permanent ...
متن کاملDevelopment of Eimeria nieschulzi (Coccidia, Apicomplexa) Gamonts and Oocysts in Primary Fetal Rat Cells
The in vitro production of gametocytes and oocysts of the apicomplexan parasite genus Eimeria is still a challenge in coccidiosis research. Until today, an in vitro development of gametocytes or oocysts had only been shown in some Eimeria species. For several mammalian Eimeria species, partial developments could be achieved in different cell types, but a development up to gametocytes or oocysts...
متن کاملThe immunizing potential of sporulated oocysts of Eimeria nieschulzi exposed to heat and Co-60 gamma-radiation.
Sporulated oocysts of Eimeria nieschulzi Dieben 1924, a rat coccidium, were exposed to radiation, heat, or both in an effort to attenuate the parasite. Moderate levels of each treatment or combination thereof attenuated the parasite, reduced pathogenesis (as judged by oocyst discharge during primary infection), and produced immunity to challenge when the oocysts were subsequently inoculated int...
متن کاملIdentification and analysis of Eimeria nieschulzi gametocyte genes reveal splicing events of gam genes and conserved motifs in the wall-forming proteins within the genus Eimeria (Coccidia, Apicomplexa)
The genus Eimeria (Apicomplexa, Coccidia) provides a wide range of different species with different hosts to study common and variable features within the genus and its species. A common characteristic of all known Eimeria species is the oocyst, the infectious stage where its life cycle starts and ends. In our study, we utilized Eimeria nieschulzi as a model organism. This rat-specific parasite...
متن کاملFixing coccidian oocysts is not an adequate solution to the problem of preserving protozoan type material.
Fresh (36 days old) sporulated oocysts of Eimeria nieschulzi were divided into 7 groups. Control oocysts were maintained at 23 C in 2% aqueous (w/v) K2Cr2O7. The 6 experimental groups were mixed with either Bouin's solution, 10% aqueous (v/v) buffered formalin, Karnovsky's solution, glutaraldehyde, paraformaldehyde, or 70% aqueous (v/v) ethanol (EtOH). After 115 days, oocysts from all 7 groups ...
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ورودعنوان ژورنال:
- The Journal of parasitology
دوره 64 1 شماره
صفحات -
تاریخ انتشار 1978